Chemo-enzymatic synthesis of chiral precursor molecules with chiral ring hydroxyenone and acetoxyenone structures

A biocatalytic transformation has the potential to perform organic reactions that are quite challenging achieve with synthetic chemistry. They also catalyze these a chemo and enantio selective manner. The discovery development of new chemoenzymatic methods for synthesis chiral structures is essential production wide range bioactive compounds. In this study, two important pharmaceutical precursors were synthesized chemoenzymatically subjected conversions different dehydrogenases. One compound an α-acetoxy enone structure 4-methoxy-2-oxacyclohex-3-enyl acetate other α-hydroxy ketone 6-hydroxy-3-methoxycycyclohex-2-enone. To obtain precursors, 3-methoxy-cyclohex-2-enone was prepared using 1,3-diketone as starting material. After obtaining material, by chemical acetylation enzymatic deacetylation. products elucidated NMR analysis. addition, reduction involving enzymes galactitol dehydrogenase (GatDH), shikimate (SDH) diaphorase carried out products.